ABSTRACT
Objective To explore the effect of comprehensive nursing intervention on the rehabilitation of patients with schizophrenia,and to provide reference for clinical nursing.Methods Sixty-seven long-term hospitalized patients with schizophrenia received 6 months comprehensive nursing intervention.The instruments of the nurse observation scale for inpatient evaluation(NOSIE),insight and treatment attitude questionnaire (ITAQ),scale of social function in psychosis inpatients (SSPI) were administered both at the start and the end of intervention.Result After 6 months of treatment,the scores on mental disorder,recovery of social ability and treatment compliance and recovery or selfconcicousness increased as compared with pre-intervention (all P＜0.001).Conclusion The comprehensive nursing intervention can improve the mental symptoms and social function,alleviate their depression and promote mental rehabilitation of the patients with schizophrenia.
ABSTRACT
Radiation sensitive protein 23 (RAD23) is a nucleotide excision repair (NER) protein that plays an important role in Ubiquitin-proteasome pathway (UPP). Schistosoma japonicum radiation sensitive protein23 (SjRAD23) cDNA sequences were amplified by PCR and cloned into pET28a (+) vector to construct recombinant expression plasmid pET28a(+)-SjRAD23. The recombinant protein was expressed as both inclusion bodies and the supernatant in Escherichia coli BL21 (DE3) cell. Immunofluorescence observation shows that SjRAD23 was mainly distributed on the tegument surface of the worms. ELISA assay reveals that specific IgG, IgG1 and IgG2a antibodies could be detected in the sera of rSjRAD23 immunized mice. Western blotting analysis shows that the recombinant SjRAD23 could be recognized by serum specific to soluble adult worm antigen of S. japonicum. BALB/c mice vaccinated with rSjRAD23 combined with 206 adjuvant revealed 35.94% worm reduction and 40.59% liver egg reduction when compared with that of the adjuvant control